Guide · Chapter 4 of 8 · Measure

Setting up a step test

Starting load, stage increment, stage duration and breaks. With ready-made protocols for treadmill, track and bike.

7 min readLast updated: November 2026
In brief
  • The workload increases in stages of equal length. At the end of each stage you measure lactate and heart rate.
  • A stage lasts at least 3 minutes; a test has at least 5 stages.
  • Start easy. The first stages must be clearly below your threshold.
  • Keep the protocol, breaks and conditions the same for every test. Only then are tests comparable.

The step test is the standard method in lactate diagnostics. It is not rocket science, but it does not forgive sloppiness with the protocol. This chapter shows you how to set it up and gives you ready-made protocols to work with.

The principle

You run, cycle or row at a fixed workload, hold it for a few minutes and then increase it by a fixed increment. At the end of each stage, a drop of blood is measured and the heart rate noted. This is how your lactate curve takes shape, point by point.

Structure of a step test on the bikeSeven stages of three minutes each, from 100 to 280 watts in increments of 30 watts. Measurements are taken at the end of each stage, plus at rest and during recovery.01002003000369121518212427Power in wattsTime in minutes100Stage 1: 100 watts, measurement after 3 minutes130Stage 2: 130 watts, measurement after 6 minutes160Stage 3: 160 watts, measurement after 9 minutes190Stage 4: 190 watts, measurement after 12 minutes220Stage 5: 220 watts, measurement after 15 minutes250Stage 6: 250 watts, measurement after 18 minutes280Stage 7: 280 watts, measurement after 21 minutesResting value before the startResting valueRecovery, 1st minute after stoppingRecovery, 3rd minute after stoppingRecovery, 5th minute after stoppingRecoveryBlood drop and heart rate at end of each stage
Example for the bike: start at 100 watts, 30 watts more per stage, 3 minutes per stage. Plus a resting value before the start and three values during recovery.

Why stages and not a steadily rising ramp? Lactate needs time. After a change in workload, it takes 3 to 5 minutes for the level in the blood to settle. In a ramp, lactate lags behind the workload. In a step test, each workload has exactly one lactate value.

The five variables

1. Starting load

Start so easy that you could hold a conversation. The first two to three stages should be clearly below your aerobic threshold. Only then does the curve show where lactate begins to rise. If in doubt, start one stage lower.

2. Stage increment

The increment from stage to stage depends on the sport and your performance level. It stays the same throughout the test. Increments that are too large give too few points on the curve; increments that are too small make the test unnecessarily long.

3. Stage duration

At least 3 minutes; 3 to 5 minutes is usual. The duration has consequences for the analysis: the shorter the stages, the lower the lactate values at the same power output. That is why every threshold model comes with a protocol that suits it. More on this in Chapter 5.

4. Break

When running, you need a short break for blood sampling. It should last about 30 seconds and be the same length at every stage. On an ergometer, samples can be taken from the earlobe without interruption.

5. Number of stages

Plan for at least 5 stages, preferably 6 to 8. If your test has fewer, the start was too high or the increment too large.

Ready-made protocols

The following protocols are proven starting points. Choose the start and the increment so that you end up with 6 to 8 stages.

Treadmill

Performance levelStartIncrementStage duration
Beginners6 km/h1 km/h3 min
Recreational runners8 km/h1 to 2 km/h3 min
Competitive athletes10 km/h2 km/h3 min
Classic, after Mader7.2 km/h (2.0 m/s)1.44 km/h (0.4 m/s)5 min

Treadmill gradient 1%, break for blood sampling about 30 seconds.

Track and field test

On a 400-metre track you run a fixed distance per stage. With 1,200 metres, i.e. three laps, even the fastest stage still lasts long enough. You set the pace using the lap time, a GPS watch or a pacing bike.

SpeedTime per kmTime per 400 mStage over 1,200 m
8 km/h7:30 min3:00 min9:00 min
10 km/h6:00 min2:24 min7:12 min
12 km/h5:00 min2:00 min6:00 min
14 km/h4:17 min1:43 min5:09 min
16 km/h3:45 min1:30 min4:30 min
18 km/h3:20 min1:20 min4:00 min

Times rounded to whole seconds. Alternatively, you run every stage for the same length of time, for example 3 minutes, and note the distance. In the field, the rule is: flat course, little wind, same conditions as in the last test.

Bike

Performance levelStartIncrementStage duration
Beginners50 to 75 watts25 watts3 min
Trained athletes80 to 120 watts30 to 40 watts3 min
Example from the quick-start guide100 watts25 watts3 min

Keep your cadence the same throughout the test. Your own pedals, your usual riding position and a fan help.

Rowing

The same rules apply on the rowing ergometer: start at 75 to 100 watts, or at 150 watts for trained athletes, then 25 to 50 watts more per stage, 3 minutes per stage.

What a record sheet looks like

The header of the sheet contains name, date, time, location, sport, meter, sampling site, temperature and body weight. Below that comes one row per stage. Here is a completed running test as an example.

StageSpeedDurationHeart rateLactatePerceived effort
Rest––621.1–
18 km/h3 min1281.4very light
210 km/h3 min1421.3light
312 km/h3 min1551.6comfortable
414 km/h3 min1672.4somewhat hard
516 km/h3 min1784.3hard
618 km/h3 min1878.2very hard
Recovery 3 min––1318.9–

Example values, lactate in mmol/L. If someone stops early in the last stage, you note the time reached. The stage is only included in the analysis if at least half of it was completed.

It is normal for the second value here to be slightly below the first. In well-trained athletes, lactate often drops slightly at the beginning before it rises.

Preparation

  • Start healthy: Only test when you feel healthy. If you have pre-existing conditions or risk factors, a medical examination should come before your first exercise test.
  • Two days before: no competition, no hard training, eat normally, drink enough.
  • On test day: eat as you normally do and rich in carbohydrates, with the last meal two to three hours before the test. After that, water only.
  • What to bring: your usual shoes or your own pedals, a heart rate chest strap, the record sheet from your last test.
  • Room: 18 to 22 °C and a fan are ideal.

The procedure on test day

  1. Measure the resting value

    Sit for a few minutes, then note your resting lactate and resting heart rate.

  2. Complete the stages

    Set the workload, hold it for the whole stage, take the drop of blood and the heart rate at the end, then move on to the next stage. How to take a clean sample is explained in Chapter 3.

  3. Stop

    The test ends when you can no longer hold a stage. For guiding your training, you do not have to go to complete exhaustion.

  4. Measure recovery

    Spin your legs or walk easily and measure again after 1, 3 and 5 minutes. After you stop, lactate initially keeps rising and then falls.

  5. Analyse

    The values go into the Lactate Express App or into WinLactat.

Stop immediatelyIf you experience dizziness, chest pain, breathlessness that feels unusual, or if you suddenly feel unwell, end the test immediately. Safety comes before the reading.

Typical mistakes

  • Started too high: The curve begins when lactate is already rising, and the aerobic threshold cannot be determined.
  • Stages too short: Lactate has not settled, and the thresholds appear to shift upwards.
  • Uneven breaks: 20 seconds one time, a minute the next. That shifts the values.
  • Too few stages: A reliable curve cannot be fitted to three or four points.
  • Protocol changed: A test with 3-minute stages and one with 5-minute stages are not comparable.
  • Lactate in a ramp test: For lactate thresholds you need stages.

Repeating and comparing

A single test shows where you stand. Only the next one shows whether your training is working. To be able to compare tests, you keep everything the same: protocol, time of day, preparation, sampling site, meter. For triathletes there is one more rule: each discipline is tested separately. Thresholds and heart rate values from the bike cannot be converted to running.

The protocols are examples from diagnostic practice and are no substitute for individual advice. If you have no experience with exercise tests, it is best to gain it first with a qualified performance diagnostics provider.